tag:blogger.com,1999:blog-5255808252082956251.post7991264481482111903..comments2024-02-16T01:48:30.016-08:00Comments on mathbionerd: Notes on: Single-cell RNA-Seq reveals dynamic, random monoallelic gene expression in mammalian cellsmathbionerdhttp://www.blogger.com/profile/17525536407206138695noreply@blogger.comBlogger4125tag:blogger.com,1999:blog-5255808252082956251.post-32241535441216331312014-01-30T05:59:58.999-08:002014-01-30T05:59:58.999-08:00Mark, I think you're right. Each analysis is i...Mark, I think you're right. Each analysis is in a single cell during extremely early development (2-cell, 4-cell, 16-cell stages). Most of this is likely just variance in transcription across single cells, because, as the authors state, the signal disappears when expression across multiple cells is considered.<br /><br />Further, as Kelkar states, and the authors recognize, the bulk of this signal is likely just transcriptional bursting. <br /><br />That's why I focused primarily the X chromosome, where this method, when considered over a whole chromosome, might actually give a picture of when whole chromosome silencing is initiated. mathbionerdhttps://www.blogger.com/profile/17525536407206138695noreply@blogger.comtag:blogger.com,1999:blog-5255808252082956251.post-56004353868524649202014-01-30T04:17:34.980-08:002014-01-30T04:17:34.980-08:00"After inferring the proportion of losses RNA..."After inferring the proportion of losses RNA molecules, the authors propose that 12-24% of genes exhibit monoallelic expression in single cells."<br /><br />I am not in the field and have a hard time evaluating their degree of certainty but I find this almost impossible to believe, as it seems like this would inevitably uncover heterozygous hits in essential genes. How much of a snapshot in time is this--if protein perdurance were substantially longer than transcript lifetime, parental alleles could be switching on and off really rapidly it might not have the effect I'd expect.Mark Phttps://www.blogger.com/profile/14462250693712068754noreply@blogger.comtag:blogger.com,1999:blog-5255808252082956251.post-4076722857090513482014-01-28T08:47:37.088-08:002014-01-28T08:47:37.088-08:00I am a little embarrassed to admit I didn't kn...I am a little embarrassed to admit I didn't know either.<br /><br />Hmm... do you know if transcriptional bursts lead to complete transcripts or partial transcripts? If they're complete transcripts including UTRs, then I don't see why they wouldn't be translated into proteins. mathbionerdhttps://www.blogger.com/profile/17525536407206138695noreply@blogger.comtag:blogger.com,1999:blog-5255808252082956251.post-91986639348016377802014-01-28T06:49:18.927-08:002014-01-28T06:49:18.927-08:00Very interesting, the transcriptional burst phenom...Very interesting, the transcriptional burst phenomenon. Expression noise in bacteria is quite well-known, but was surprised to see how widespread it seems to be in vertebrates. I wonder how well the transcriptional bursts 'translate' to bursts of protein synthesis.<br />Kelkarhttps://www.blogger.com/profile/10567805236197247624noreply@blogger.com